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Anti -FITC Mouse mAb

價格: 在線咨詢
貨號:
DRF10133
產品規格:
100 μL
Applications:
Elisa
Species:
購買數量:
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產品詳情 操作說明 注意事項

Product Information


Production Name FITC mouse monoclonal
Synonyms 5-Isothiocyanat Fluorescein,
FITC, Fluorescein
Immunogen Synthesized peptide derived from FITC
Specificity This antibody detects FITC conjunted protein or antibody.
Source Rabbit
Concentration 1 mg/ml
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.




Applications


Applications Dilution
Elisa 1: 5000-1: 10000




Background

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications. It is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. FITC is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. This mouse antibody detects the FITC only. This antibody can be coated in the plate to capture the FITC conjugated protein or antibody. Moreover, this mouse antibody used with HRP goat anti-rabbit secondary antibody, converts fluorescent signal to DAB dyeing.




Images





Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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