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產品詳情
操作說明
注意事項
Product Information
|
Description |
6 X His Tag rabbit polyclonal |
|
Synonyms |
6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His probe, His Tag, His-probe, HISTIDINE TAGGED |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to HHHHHH (6* His) |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
Applications
|
Applications |
Species |
Dilution |
Positive Tissue |
|
WB |
-- |
1: 500-1: 1000 |
His-Tag fusion protein |
Background
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His-tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.
Images
|
|
Western blot analysis of 6*His Tag (GB111251) at dilution of 1: 1000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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合肥萬物生物科技有限公司
客服熱線：400-1016-218
QQ：355185756
地址：安徽省合肥市高新區黃山路602號合肥國家大學科技園A401室