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產品詳情
操作說明
注意事項
Product Information
| Description |
Ki67 rabbit polyclonal antibody |
| Protein full name |
Proliferation marker protein Ki-67 |
| Synonyms |
MKI67, marker of proliferation Ki-67, KIA, MIB-, MIB-1, PPP1R105 |
| Immunogen | Recombinant protein corresponding to Mouse Ki67 |
| Isotype | IgG |
| Subcellular location |
Nucleus,nucleolus |
| Purity | Affinity purification |
| Predicted MW. | 359KDa |
| Observed MW. |
359KDa |
| Uniprot ID | E9PVX6 |
Applications
|
Applications |
Species |
Dilution |
Positive Tissue |
|
WB |
Human, Mouse |
1:300-1:600 |
liver, stomach, colon |
Background
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (By similarity).
Images
|
|
Western blot analysis of KI67 (GB11030) at dilution of 1: 300 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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合肥萬物生物科技有限公司
客服熱線：400-1016-218
QQ：355185756
地址：安徽省合肥市高新區黃山路602號合肥國家大學科技園A401室